N Acetyl Transferase
Request information or ask a question about N-Acetyl Transferase modification of drugs
• N-Acetyl Transferase (NAT) activity assays
• LC/MS/MS detection of acetylated-drug conjugates
• Comparison to prototypical human NAT inducers
• Phase II enzyme
Many CROs are experienced in assays to detect N-Acetyl Transferase (NAT) induction and acetylated-drug conjugates for pre-clinical compounds. CROs typically utilize standard acetyl-transferase assays in comparison to known standards to detect initiation of NAT activity in hepatocytes. LC/MS/MS can be used to directly analyze acetylated-drug adducts after incubation with hepatocytes.
NAT enzymes (2 known) play a key role in reduction of cellular toxicity via detection of contaminants and direct conjugation of acetyl groups to many arylamine and hydrazine drugs. NATs are found in the cytosol and are well known to participate in the clearance of anti-cancer drugs and other xenobiotics that can lead to multifactorial adverse effects and xenobiotic-related diseases.
CROs will typically work with your team to develop proprietary methods that best suit your study needs.
How to Ask a Question
If you want to request technical information, ask a question or get expert feedback on your latest "crazy" idea, click on the orange Request Info button. Type or paste in your question, upload a supporting document (if desired) and choose from a comprehensive list of vendors in this research area. Once you submit your question or request, you will be able to view responses and continue a private conversation with any expert that responds.
