Cell line expansion and ascites production using 5, 10, 15 or 20 BALB/c mice

Large scale expression, extraction, and purification of at least 1 g of recombinant antibody from your mammalian cells

Cloning, construction with hIgG constant regions, production and purification of 100 µg antibody in mammalian cells

Production of cell culture supernatant from a antibody-producing hybridoma cell line

Generation of two stable cell pools and five individual cell clones stably expressing your antibody